LactoSpore® and DE111®: The Clinically Studied Probiotic Strains in Lemme Debloat Gummies
By Lemme Editorial Team
Reviewed by Dr. Kathleen Valenton, MD, OB/GYN, Board-Certified Physician, Cedars-Sinai Medical Center · Estimated read time: 6 minutes
When it comes to probiotic gummies for bloating, ingredient quality and clinical evidence behind each strain matter more than marketing claims. Lemme Debloat Gummies are formulated with two clinically studied, spore-forming probiotic strains — LactoSpore® (Bacillus coagulans MTCC 5856) and DE111® (Bacillus subtilis) — paired with Prebiotic XOS to feed and support the beneficial bacteria in your gut.*
This article breaks down the science behind both strains: what they are, how they work, and what the human clinical research shows.*
What Are Spore-Forming Probiotics — And Why Does It Matter?
Spore-forming probiotics form protective endospores that may help them survive the stomach environment and reach the gut, where they become active. Not all probiotic strains share this property — some may be more sensitive to stomach acid, which can affect how much of the probiotic reaches the gut.
Both LactoSpore® and DE111® form protective endospores that may help them survive the acidic environment of the stomach and bile salts in the small intestine, germinating and becoming active once they reach the gut. This survivability has been evaluated in human research. A crossover, double-blind, placebo-controlled study in ileostomy patients found that DE111® was detectable in the human small intestinal tract within three hours of ingestion, with spore and vegetative cell concentrations increasing substantially in the hours following. [5]
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Key Takeaway: LactoSpore® and DE111® — the two probiotic strains in Lemme Debloat Gummies — are spore-forming, meaning they form protective endospores that may help them survive the stomach environment and reach the gut. Spore-forming probiotics are designed for digestive survivability, which is an important consideration when choosing a daily probiotic for bloating support.* |
LactoSpore® (Bacillus coagulans MTCC 5856): What the Research Shows
LactoSpore® is a clinically studied, spore-forming probiotic strain that has been evaluated in multiple randomized, placebo-controlled human trials specifically for bloating and gas. It is a patented strain of Bacillus coagulans MTCC 5856, developed and clinically studied by the Sami-Sabinsa Group over more than 20 years of research. [3] It produces the L(+) form of lactic acid, which may help support a balanced gut environment, and has been well-tolerated with no significant adverse events reported across multiple clinical trials.*
Clinical Evidence for Bloating and Gas
A multicenter, randomized, double-blind, placebo-controlled trial published in 2023 evaluated LactoSpore® in 70 adults with functional gas and bloating over 30 days. GSRS indigestion scores in the probiotic group decreased from 8.91 to 3.06 (p < .001), compared to minimal change in the placebo group. Scores for abdominal pain, reflux, diarrhea, and constipation also improved significantly in the LactoSpore® group. [1, 2]
Additional Digestive Research
A separate randomized, double-blind, placebo-controlled study evaluated LactoSpore® in adults with functional digestive symptoms. Participants showed significant decreases (p < 0.01) in bloating, abdominal pain, and stool frequency compared to placebo, with improvements in overall digestive quality of life. [4]
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EXPERT PERSPECTIVE “LactoSpore® stands out to me because the clinical evidence is specific to the outcomes patients actually care about — gas, bloating, and abdominal discomfort. Too often, probiotic research is conducted in very specific disease populations and extrapolated broadly. The LactoSpore® bloating study was conducted in otherwise healthy adults with functional symptoms, which is much closer to the population actually reaching for a daily debloating supplement.” — Dr. Kathleen Valenton, MD, OB/GYN, Cedars-Sinai Medical Center* |
DE111® (Bacillus subtilis): What the Research Shows
DE111® is a spore-forming probiotic strain of Bacillus subtilis that has been evaluated in randomized, placebo-controlled clinical trials for digestive health and gut regularity in both adults and children. It has a long history of safe human consumption — found naturally in natto, a traditional Japanese fermented food — and is well-tolerated with no serious adverse events reported across multiple studies. [5, 6]
Clinical Evidence for Digestive Health
A 105-day randomized, double-blind, placebo-controlled study in 50 adults with occasional constipation and/or diarrhea found that DE111® participants showed statistically significant improvements in stool consistency compared to placebo, while the placebo group showed no significant change. [7]
Gut Survivability: Evaluated in Human Research
A crossover, double-blind, placebo-controlled ileostomy study evaluated whether DE111® reaches the human small intestinal tract — with both spores and vegetative cells detected within three hours of ingestion, increasing substantially over eight hours. [5]
The Role of Prebiotic XOS in Lemme Debloat Gummies
Prebiotic XOS (Xylooligosaccharides) serves as a food source for the beneficial bacteria that LactoSpore® and DE111® help establish in the gut. Lemme Debloat Gummies include XOS sourced from non-GMO plant fibers — a prebiotic fiber that has been studied to support the growth of beneficial gut bacteria, helping them survive and stay active.*
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EXPERT PERSPECTIVE “What I find compelling about the formulation in Lemme Debloat Gummies is the thoughtfulness behind strain selection. LactoSpore® has been studied for bloating and gas directly. DE111® supports gut regularity and has been evaluated for gut survivability in humans. And XOS feeds the beneficial bacteria both strains help establish. Each ingredient has a specific, evidence-informed role, which is what I look for when recommending any supplement to my patients.” — Dr. Kathleen Valenton, MD, OB/GYN, Cedars-Sinai Medical Center* |
Frequently Asked Questions
What probiotics are in Lemme Debloat Gummies?
Lemme Debloat Gummies contain two clinically studied, spore-forming probiotic strains: LactoSpore® (Bacillus coagulans MTCC 5856), which has been evaluated in multiple randomized, placebo-controlled human trials specifically for bloating and gas relief, and DE111® (Bacillus subtilis), a strain studied in adults and children for digestive health and gut regularity. Both strains are paired with Prebiotic XOS (Xylooligosaccharides) — a plant-sourced prebiotic that feeds and supports the beneficial bacteria both strains help establish.*
What does LactoSpore® do for bloating?
LactoSpore® (Bacillus coagulans MTCC 5856) has been the subject of multiple randomized, placebo-controlled human trials over more than 20 years of research. In a multicenter, randomized, double-blind, placebo-controlled study in 70 adults with functional gas and bloating, daily LactoSpore® supplementation was associated with a significant reduction in indigestion scores after 30 days (8.91 to 3.06, p < .001) compared to minimal change in the placebo group. Scores for abdominal pain, reflux, diarrhea, and constipation also improved significantly in the LactoSpore® group. LactoSpore® produces the L(+) form of lactic acid, which may help support a balanced gut environment.*
How long does it take for Lemme Debloat Gummies to work?
In the clinical study on LactoSpore®, significant improvements in bloating and gas were observed after 30 days of daily use. Lemme recommends taking Debloat Gummies daily for at least 6 weeks for optimal probiotic benefit. As with any supplement, individual results may vary based on your unique gut health baseline.*
Why do the probiotics in Lemme Debloat Gummies form spores?
Some probiotic strains may be sensitive to the acidic environment of the stomach, which can affect how much of the probiotic reaches the gut. Spore-forming probiotics like LactoSpore® and DE111® form protective endospores that may help them survive the stomach environment and bile salts in the small intestine, germinating and becoming active once they reach the gut. For DE111®, survivability in the human small intestinal tract has been evaluated in a clinical study — with both spores and vegetative cells detected within three hours of ingestion.*
About Lemme Debloat Gummies
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Lemme Debloat Gummies ✓ LactoSpore® (Bacillus coagulans MTCC 5856) ✓ DE111® (Bacillus subtilis) ✓ Prebiotic XOS (Xylooligosaccharides) ✓ Vegetarian · Gluten-Free · Non-GMO · No Artificial Dyes or Sweetners ✓ Blackberry flavor · Made in the USA in a cGMP certified facility 2-in-1: Clinically Studied Probiotics + Prebiotic |
Citations
[1] Majeed M, et al. (2023). The effects of Bacillus coagulans MTCC 5856 on functional gas and bloating in adults. PubMed. https://pubmed.ncbi.nlm.nih.gov/36862903/
[2] PMC Full Text. https://pmc.ncbi.nlm.nih.gov/articles/PMC9982755/
[3] LactoSpore Clinical Research Overview. Sami-Sabinsa Group. https://lactospore.com/research/clinical
[4] LactoSpore® IBS and microbiome study. Sami-Sabinsa Group. https://lactospore.com/research/clinical
[5] Colom J, et al. Presence and Germination of the Probiotic Bacillus subtilis DE111® in the Human Small Intestinal Tract. Frontiers in Microbiology, 2021. https://www.frontiersin.org/articles/10.3389/fmicb.2021.715863/full
[6] Paytuví-Gallart A, et al. (2022). In vitro and in silico assessment of probiotic and functional properties of Bacillus subtilis DE111®. Frontiers in Microbiology. https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2022.1101144/full
[7] Cuentas AM, et al. The Effect of Bacillus subtilis DE111 on the Daily Bowel Movement Profile. Journal of Probiotics & Health. https://www.longdom.org/open-access/the-effect-of-embacillus-subtilisem-de111-on-the-daily-bowel-movement-profile-for-people-with-occasional-gastrointestina-36720.html
[8] Freedman KE, et al. (2021). Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111. International Journal of Molecular Sciences. https://pmc.ncbi.nlm.nih.gov/articles/PMC7957723/
About the Expert Reviewer
Kathleen Valenton, MD is a double board-certified OB/GYN at Rodeo Drive Women's Health Center in Beverly Hills, CA, with over a decade of experience in women's health. She completed her internship and residency at Cedars-Sinai Medical Center — where she remains an attending physician — after earning her Medical Degree from the University of Vermont College of Medicine and dual Bachelor's and Master's degrees in Psychology from UC Berkeley. Dr. Valenton is also the Chief Medical Officer at Lemme. Her clinical research interests span mental health, hormonal health, infertility, PCOS, and menopause. Dr. Valenton is known for her expertise in pelvic pain, infertility evaluations, and minimally invasive surgery, and remains actively involved in resident education and physician-nursing collaboratives at Cedars-Sinai.
*These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Individual results may vary.
